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subtitle: Working to create a world where no animals suffer in a laboratory
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Alternatives to animal testing
Non-animal methods are often cheaper, quicker and more effective
Replacing animal tests does not mean putting human patients at risk. It also does not mean halting medical progress. Instead, replacing animals used in testing will improve the quality as well as the humanity of our science.
Thankfully, the development of non-animal methods is growing, and fast. Due to innovations in science, animal tests are being replaced in areas such as toxicity testing, neuroscience and drug development. But much more needs to be done.
Once new non-animal methods have been developed, there are often massive bureaucratic hurdles to implementing and enforcing their use. One of the most important jobs the Cruelty Free International science team does is encourage regulators to accept and promote the use of non-animal methods to replace animal testing.
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Types of non-animal methods
Cell cultures
Almost every type of human and animal cell can be grown in the laboratory. Scientists have even managed to coax cells to grow into 3D structures, such as miniature human organs, which can provide a more realistic way to test new therapies.
Human cells have been used to create innovative little devices called “organs-on-chips”. These can be used instead of animals to study biological and disease processes, as well as drug metabolism. Devices have already been produced that accurately mimic the lung, heart, kidney and gut. The ultimate goal is to use these chips to create a whole “human-on-a-chip”.
Cell cultures have been central to key developments in areas such as cancers, sepsis, kidney disease and AIDS, and are routinely used in chemical safety testing, vaccine production and drug development.
Human tissues
Both healthy and diseased tissues donated from human volunteers can provide a more relevant way of studying human biology and disease than animal testing.
Human tissue can be donated from surgery (e.g. biopsies, cosmetic surgery and transplants). For example, skin and eye models made from reconstituted human skin and other tissues have been developed and are used to replace the cruel rabbit irritation tests.
Human tissue can also be used after a person has died (e.g. post-mortems). Post-mortem brain tissue has provided important leads to understanding brain regeneration and the effects of Multiple Sclerosis and Parkinson’s disease.
Computer models
With the growing sophistication of computers, the ability to “model” or replicate aspects of the human body is ever more possible.
Computer models of the heart, lungs, kidneys, skin, digestive and musculoskeletal systems already exist. They can be used to conduct virtual experiments based on existing information and mathematical data.
There are also data mining tools that can help make predictions about the likely hazard of one substance based on existing data from other, similar substances.
Volunteer studies
Advances in technology have allowed for the development of sophisticated scanning machines and recording techniques that can be used to study human volunteers safely.
Brain imaging machines that can ‘see’ inside the brain can be used to monitor the progression and treatment of brain disease. They can help researchers understand the causes by comparing with healthy volunteers.
An innovative technique called microdosing can also be used in volunteers to measure how very small doses of potential new drugs behave in the human body. These microdoses are radio-labelled, injected into human volunteers and measured (usually in blood samples) using a very sensitive measuring device called an accelerator mass spectrometer.
Less high-tech studies for nutrition, drug addiction and pain can also be carried out on consenting humans in the interest of advancing medical science. These studies can help replace animal tests and come with the obvious advantage that people are able to explain how they are feeling.
Medical breakthroughs using humans
- We are told that insulin therapy would not have been discovered unless animal researchers had removed the pancreas from dogs in the 1920s. But the important clues actually came much earlier from observations of human patients.
- Brain surgery in Parkinson’s patients identified the best place for Deep Brain Stimulation electrodes to be placed in the brain to improve symptoms, decades before the “discovery” in monkeys.
- Alois Alzheimer first described the main features of Alzheimer’s disease in 1906 by studying brain segments from patients after they had died.
- Human population studies led to the discovery that smoking causes cancer. Smoking does not cause cancer in mice and rats.
- An Australian doctor used himself in an experiment to discover the main cause of stomach ulcers. He drank a culture of bacteria and became sick before curing his symptoms with antibiotics.
- A German chemist tested the effects of aspirin on himself after an accidental discovery that it helped relieve pain in a patient with toothache.
- The anaesthetic effect of laughing gas was discovered when someone accidentally cut their leg while under the influence of the gas. An American dentist then confirmed the effects on himself while having a tooth removed.
Non-animal methods perform better than animal tests
- Crude skin allergy tests in guinea pigs and mice only predict human reactions 72% and 74% of the time, respectively. But approaches combining chemistry- and cell-based alternative methods have been shown to accurately predict human reactions up to 85% of the time.
- The notorious Draize skin irritation test in rabbits can only predict human skin reactions 60% of the time. But methods using reconstituted human skin are up to 86% accurate.
- Tests on animals to find out if chemicals or drugs may harm the developing baby can only detect 60% of dangerous substances. But a non-animal test using human stem cells has 93% sensitivity at detecting substances known to cause developmental problems.
- Cruel and unreliable shellfish toxin testing using live mice has now been fully replaced with a far superior analytical chemistry method that is better at protecting humans from shellfish poisoning.
The science relating to animal experiments can be extremely complicated and views often differ. What appears on this website represents Cruelty Free International expert opinion, based on a thorough assessment of the evidence.
FIND OUT MORE ABOUT ANIMAL TESTING
Alternatives to animal tests are often cheaper, quicker and more effective.
subtitle: Alternatives to animal tests are often cheaper, quicker and more effective.
Science Page
Established in 1898, Cruelty Free International is firmly rooted in the early social justice movement and has a long and inspiring history.
Our History
subtitle: Established in 1898, Cruelty Free International is firmly rooted in the early social justice movement and has a long and inspiring history.
Millions of animals are used and killed in the name of progress every year.
Facts and figures on animal testing
subtitle: Millions of animals are used and killed in the name of progress every year.
Animals used in laboratories are deliberately harmed, not for their own good, and are usually killed at the end of the experiment.
What is animal testing?
subtitle: Animals used in laboratories are deliberately harmed, not for their own good, and are usually killed at the end of the experiment.
Animal experiments are cruel, unreliable, and even dangerous.
Arguments against animal testing
subtitle: Animal experiments are cruel, unreliable, and even dangerous.
Animal testing is carried out in a wide range of areas, including biological research, and testing medicines and chemicals.
Types of animal testing
subtitle: Animal testing is carried out in a wide range of areas, including biological research, and testing medicines and chemicals.
Science Publications
Alternatives to animal experiments
Millions of dogs, monkeys, mice and other animals currently suffer in U.S. laboratories . But non-animal alternatives will one day completely replace experiments on animals. This will be a game-changer not just for animals, but for human health.
Our mission is to accelerate this change so that animals in laboratories no longer suffer when superior methods for developing medicines, understanding diseases, and ensuring that products are safe, are within our reach.
We do this by:
- advocating for increased funding at the state and federal levels to develop non-animal technologies;
- urging the government to allow companies to use these technologies instead of experimenting on animals;
- and asking laboratories to use these technologies whenever possible.
Outdated animal experiments are hindering progress
We believe that a reliance on the outdated animal experiments that are in use today is actually hindering potential cures and breakthroughs in medicine while also preventing scientists from understanding how products like weed killer or toilet bowl cleaner and bleach could be negatively affecting people’s health.
Because animals and humans are very different, results from animal tests are often not applicable to people, yet we continue these largely futile attempts to understand the human body by experimenting on animals. Extensive evidence demonstrates that results from toxicity tests in animals—tests that attempt to determine how a substance such as a medicine or chemical may negatively affect humans—often don’t accurately predict toxicity in humans. In fact, approximately 90% of medicines ultimately fail in human trials following animal tests.
And the inverse also occurs: A drug found to be toxic to animals will likely never advance to human clinical trials, meaning that potentially lifesaving medicines are not pursued.
In contrast to animal experiments, non-animal alternatives employ cutting-edge methods that use human cells or are based on human data to more accurately and effectively predict how the human body will respond to drugs, chemicals and treatments.
Some of these modern approaches even use a patient’s own cells to test treatments or develop drugs based on a person’s unique genetic makeup, an approach known as personalized medicine. Unlike animal experiments, these methods represent the very latest techniques that science has to offer, provide countless possibilities to improve our understanding and treatment of the human body, and will only continue to improve over time. These non-animal methods are also typically faster and often less expensive than animal tests.
Stand with us to demand that the federal government, state governments, companies and universities stop relying on outdated animal experiments.
What alternatives are there to testing on animals?
Here are just some of the human health conditions currently benefitting from advanced non-animal technologies, with no animals harmed in the process:
Cancer: A lung organ-on-a-chip —a tiny 3D chip created from human cells that looks and functions like a miniature human organ—showed that the fluid buildup in the lungs caused by a drug frequently used by cancer patients was triggered by a patient’s lungs expanding and contracting, a finding that would not have been possible in animal experiments because researchers can’t stop and restart an animal’s lungs. The organ-on-a-chip was then used to test for drugs that would reduce the fluid buildup.
Cystic fibrosis: Organoids —3D replicas of human organs were created from the intestinal cells of a person with cystic fibrosis and were then used to test various drugs to determine which drug would be most effective in that person.
Chronic inflammatory demyelinating polyneuropathy (CIDP): A nervous system organ-on-a-chip using human cells and blood samples was created to show that muscle weakness in people with CIDP—a rare autoimmune condition—was caused by a component in the blood causing nerve damage. The results allowed scientists to get approval for a human clinical trial for a drug to treat the condition in people with CIDP. Rare conditions like CIDP can’t be studied using animal experiments because scientists aren’t able to recreate these diseases in animals, but the development of organs-on-chip models using cells from patients will help scientists to understand the cause and progression of these diseases and test possible treatments.
Skin allergies : A series of non-animal tests examining how human cells react to chemicals and how those chemicals interact with other substances is being used to determine if ingredients in everyday products such as laundry detergent, body lotion and drain cleaner will trigger an allergic reaction in human skin. These non-animal approaches have been proven to be more accurate than the outdated tests on guinea pigs and mice that are still used .
Zika virus: Brain organoids created with human cells proved that the Zika virus was causing microcephaly (small head size) in babies born to mothers who were infected with the virus. This discovery would not have been possible in animal experiments due to differences in animal and human brain structure. Scientists then used human brain organoids to test drugs that could be used to prevent or reduce the damage caused by microcephaly.
Heart arrythmias : Computer models using recordings from human heart activity are being used to test the adverse effects of potential drugs. Many drugs that are successfully tested in animals fail in human clinical trials because of dangerous effects on the human heart. This approach allows researchers to screen out potentially dangerous drug compounds before they are tested in humans—without using animals.
Autism spectrum disorder (ASD) : Scientists are using non-embryonic stem cells from the discarded baby teeth of children with ASD to create nerve cells , which are then used to study how the brains of children with ASD are different.
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